Facilities

• Magnetic Resonance Instrumentation in the NMR Service Center managed by Dr. V.P. Chacko

  • Bruker Avance 400 MHz NMR Spectrometer

  The spectrometer is built around an Oxford magnet with a field strength of 9.4 T and a vertical bore with a bore size of 8.9 cm. It was upgraded recently with a Bruker Avance console. It is equipped with actively shielded, water-cooled gradients which can produce magnetic field gradients of up to 100 G/cm in 3 directions.
  The spectrometer is suitable for spectroscopic and microimaging studies. Applications include cell perfusion and diffusion tensor imaging studies, as well as in vivo mouse studies.
  View the Bruker Avance 400 NMR Spectrometer

  • Bruker Avance 500 MHz NMR Spectrometer

  The NMR spectrometer consists of a vertical bore magnet (Oxford, bore size of 8.9 cm, field strength of 11.8 T) interfaced to a Bruker Avance console. This instrument allows high resolution NMR spectroscopy studies as well as microimaging. Applications include cell extract, cell perfusion, and diffusion tensor imaging studies, as well as in vivo mouse studies.

  The NMR spectrometer features three available actively-shielded, water-cooled gradient sets:

  • Mini0.5 with a maximum gradient strength of 30G/cm
  • Micro2.5 with a maximum gradient strength of 150 G/cm
  • Micro5 with a maximum gradient strength of 300 G/cm

  The spectrometer is equipped with Bruker-built microimaging probes allowing sample sizes of 2 mm upto 30 mm. Additionally, several Bruker-built probes are available to perform multinuclear NMR spectroscopy studies on 5 mm and 10 mm samples. This instrument also features a Bruker-built, 5-mm, high-resolution, triple-nuclei probe with in-built triple-axis gradients of maximal 40 G/cm.
  View the Bruker Avance 500 NMR Spectrometer

  • 4.7T Bruker Biospec^Avance Small Animal MR Imaging System

  This MR spectrometer has an intermediate bore size (40 cm) horizontal magnet with a field strength of 4.7 T (corresponding to a proton frequency of 200 MHz). It is used for proton as well as multinuclear imaging and spectroscopic imaging of mice, rats, rabbits or cats. This MR spectrometer features a Bruker Biospec^Avance 4.7/40 console. The spectrometer is equipped with a actively-shielded, water-cooled Bruker BGA-12 gradient set with a maximum gradient strength of 40 G/cm.
  View the 4.7 T Spectrometer

• Equipment in our In Vivo Cellular Molecular Imaging Center

  The imaging suite in our In Vivo Molecular Imaging Center is currently being set-up. It is a barrier facility that currently includes an In Vivo Bioluminescence / Fluorescence System from Xenogen for rodents, a Small Animal PET imaging system, a Small Animal SPECT/CT imaging system, a FAXITRON system, a Ultrasound system, and a horizontal bore 9.4 T Bruker Biospec MR Imager.

• Laboratories equipped for Cell Culture, Molecular Biology, and Wet Chemistry

• Three Biosafety Level II Cabinets
• Three Incubators for mammalian cell culture and one incubator for yeast culture
• Mammalian Cell Lines, Human and Animal
• Yeast Culture
• Bacteria Culture
• Three liquid nitrogen storage containers, several freezers and refrigerators
• Cold Room
• Gel Electrophoresis
• Western & Northern Blotting Capabilties
• Vis Microplate Reader
• Bright-field Fluorescence Microscopes (Nikon)
• In vivo Fluorescence Imaging System
• UV/Vis Spectrophotometer
• Nano-Drop
• Two Fume Hoods
• High speed Sorvall Centrifuge, several smaller centrifuges (for cell culture as well as Eppendorf samples)
• Sorvall Ultracentrifuge
• A ProOxC System Hypoxia Chamber from BioSpherix
• Cryostat

• Computer Equipment

  • Several Bruker Linux workstations
  • Several Sun and SGI workstations
  • MacIntoshes and Personal Computers available for the personnel in their office and in the laboratory

• Electronic and Mechanical Workshop

• Equipped with several oscilloscopes, network analyzers, coil building tools and mechanical tools
• Additionally, the workshop of the Department of Biomedical Engineering is available for more extensive mechanical work